ARG81988
Human ANP / Atrial Natriuretic Peptide ELISA Kit
Human ANP / Atrial Natriuretic Peptide ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81988-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81988-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81988-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81988-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81988-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81988-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81988-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81988-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81988-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81988-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81988-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ANP / Atrial Natriuretic Peptide in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ANP / Atrial Natriuretic Peptide |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.8%; Inter-Assay CV: 7.4% |
| Alternate Names | CDD; Natriuretic peptides A; ANF; Cardiodilatin; Cardiodilatin-related peptide; Atrial natriuretic peptide; Prepronatriodilatin; ATFB6; CDP; ATRST2; ANP; CDD-ANF; PND |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | NPPA |
| Gene Full Name | natriuretic peptide A |
| Background | The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009] |
| Function | Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. [UniProt] |
| Highlight | Related products: Atrial Natriuretic Peptide antibodies; Atrial Natriuretic Peptide ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Cleaved by CORIN upon secretion to produce the functional hormone. Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit standard curve image
ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81988 Human ANP / Atrial Natriuretic Peptide ELISA Kit User manual |
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