ARG81566
Human ANGPTL3 ELISA Kit
Human ANGPTL3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81566-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81566-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81566-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81566-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81566-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81566-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81566-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81566-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81566-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81566-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81566-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81566 Human ANGPTL3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ANGPTL3 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ANGPTL3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 156 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 312 - 20000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.4% Inter-Assay CV: 6.4% |
| Alternate Names | Angiopoietin-5; FHBL2; Angiopoietin-related protein 3; Angiopoietin-like protein 3; ANL3; ANG-5; ANGPT5 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ANGPTL3 |
| Gene Full Name | angiopoietin-like 3 |
| Background | This gene encodes a member of a family of secreted proteins that function in angiogenesis. The encoded protein, which is expressed predominantly in the liver, is further processed into an N-terminal coiled-coil domain-containing chain and a C-terminal fibrinogen chain. The N-terminal chain is important for lipid metabolism, while the C-terminal chain may be involved in angiogenesis. Mutations in this gene cause familial hypobetalipoproteinemia type 2. [provided by RefSeq, Aug 2015] |
| Highlight | Related products: ANGPTL antibodies; ANGPTL ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | O-glycosylated at Thr-226 by GALNT2; blocks processing and activation by proprotein convertases. In part proteolytically cleaved by proprotein convertases; proposed to be involved in activation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81566 Human ANGPTL3 ELISA Kit standard curve image
ARG81566 Human ANGPTL3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81566 Human ANGPTL3 ELISA Kit User's manual |
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