ARG82857
Human AKR1C3 ELISA Kit
Human AKR1C3 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG82857-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82857-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82857-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82857-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82857-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82857-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82857-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82857-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82857-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82857-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82857-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG82857 Human AKR1C3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AKR1C3 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | AKR1C3 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 78 pg/ml |
| Sample Type | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| Standard Range | 156 - 10000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.8% Inter-Assay CV: 5.8% |
| Alternate Names | Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; Testosterone 17-beta-dehydrogenase 5; EC 1.1.1.239; Dihydrodiol dehydrogenase 3; HA1753; EC 1.1.1.112; 3-alpha-HSD type II, brain; Chlordecone reductase homolog HAKRb; DD3; Indanol dehydrogenase; EC 1.1.1.188; HSD17B5; Aldo-keto reductase family 1 member C3; DDX; 17-beta-hydroxysteroid dehydrogenase type 5; hluPGFS; 3-alpha-HSD type 2; EC 1.3.1.20; EC 1.1.1.357; Prostaglandin F synthase; HAKRe; 3-alpha-hydroxysteroid dehydrogenase type 2; EC 1.1.1.64; DD-3; 17-beta-HSD 5; HAKRB; Dihydrodiol dehydrogenase type I; PGFS |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P42330 Human Aldo-keto reductase family 1 member C3 |
|---|---|
| Gene Symbol | AKR1C3 |
| Gene Full Name | aldo-keto reductase family 1, member C3 |
| Background | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9alpha,11beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and/or differentiation. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
| Function | Catalyzes the conversion of aldehydes and ketones to alcohols. Catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ) and the oxidation of 9-alpha,11-beta-PGF2 to PGD2. Functions as a bi-directional 3-alpha-, 17-beta- and 20-alpha HSD. Can interconvert active androgens, estrogens and progestins with their cognate inactive metabolites. Preferentially transforms androstenedione (4-dione) to testosterone. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82857 Human AKR1C3 ELISA Kit standard curve image
ARG82857 Human AKR1C3 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82857 Human AKR1C3 ELISA Kit User manual |
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