ARG81450
Human AHSG ELISA Kit
Human AHSG ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81450-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81450-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81450-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81450-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81450-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81450-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81450-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81450-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81450-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81450-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81450-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81450 Human AHSG ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human AHSG in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | AHSG |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.2% Inter-Assay CV: 6.2% |
| Alternate Names | A2HS; Alpha-2-HS-glycoprotein; HSGA; Ba-alpha-2-glycoprotein; AHS; FETUA; Alpha-2-Z-globulin; Fetuin-A |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | AHSG |
| Gene Full Name | alpha-2-HS-glycoprotein |
| Background | Alpha2-HS glycoprotein (AHSG), a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure. [provided by RefSeq, Jul 2008] |
| Function | Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. [UniProt] |
| Highlight | Related products: AHSG antibodies; AHSG ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Phosphorylated by FAM20C in the extracellular medium. O- and N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. N-glycan at Asn-156: Hex5HexNAc4; N-glycan heterogeneity at Asn-176: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor). [UniProt] |
Images (1) Click the Picture to Zoom In
-
ARG81450 Human AHSG ELISA Kit standard curve image
ARG81450 Human AHSG ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81450 Human AHSG ELISA Kit User's manual |
 Download
|
