ARG81565
Human ADAMTS4 ELISA Kit
Human ADAMTS4 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81565-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81565-002 | Standard | 2 X 40 ng/vial | 4°C |
| ARG81565-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81565-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81565-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81565-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81565-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81565-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81565-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81565-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81565-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81565 Human ADAMTS4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAMTS4 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ADAMTS4 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 312.5 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 625 - 40000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 4.7% Inter-Assay CV: 5.8% |
| Alternate Names | ADAMTS-4; Aggrecanase-1; ADAMTS-2; EC 3.4.24.82; A disintegrin and metalloproteinase with thrombospondin motifs 4; ADAM-TS4; ADMP-1; ADAM-TS 4 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O75173 Human A disintegrin and metalloproteinase with thrombospondin motifs 4 |
|---|---|
| Gene Symbol | ADAMTS4 |
| Gene Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 4 |
| Background | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. [provided by RefSeq, Jul 2008] |
| Function | Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site. [UniProt] |
| Highlight | Related products: ADAMTS antibodies; ADAMTS ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The precursor is cleaved by a furin endopeptidase. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81565 Human ADAMTS4 ELISA Kit standard curve image
ARG81565 Human ADAMTS4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81565 Human ADAMTS4 ELISA Kit User's manual |
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