ARG81483
Human ADAMTS13 ELISA Kit
Human ADAMTS13 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81483-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81483-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG81483-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81483-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81483-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81483-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81483-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81483-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81483-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81483-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81483-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81483 Human ADAMTS13 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAMTS13 in serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ADAMTS13 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 0.39 ng/ml |
| Sample Type | Serum, plasma (heparin, EDTA, citrate) and cell culture supernatants. |
| Standard Range | 0.78 - 50 ng/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.7% Inter-Assay CV: 6.1% |
| Alternate Names | von Willebrand factor-cleaving protease; vWF-CP; EC 3.4.24.87; ADAM-TS 13; VWFCP; A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS13; vWF-cleaving protease; ADAMTS-13; C9orf8 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q76LX8 Human A disintegrin and metalloproteinase with thrombospondin motifs 13 |
|---|---|
| Gene Symbol | ADAMTS13 |
| Gene Full Name | ADAM metallopeptidase with thrombospondin type 1 motif, 13 |
| Background | This gene encodes a member of a family of proteins containing several distinct regions, including a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. The enzyme encoded by this gene specifically cleaves von Willebrand Factor (vWF). Defects in this gene are associated with thrombotic thrombocytopenic purpura. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation. [UniProt] |
| Highlight | Related products: ADAMTS antibodies; ADAMTS ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Glycosylated. O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS13. May also be C-glycosylated on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and also N-glycosylated. These other glycosylations can also facilitate secretion. The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81483 Human ADAMTS13 ELISA Kit standard curve image
ARG81483 Human ADAMTS13 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81483 Human ADAMTS13 ELISA Kit User's manual |
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