ARG81558
Human ADAM9 ELISA Kit
Human ADAM9 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81558-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81558-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81558-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81558-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81558-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81558-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81558-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81558-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81558-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81558-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81558-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81558 Human ADAM9 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAM9 in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ADAM9 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 7.8 pg/ml |
| Sample Type | Serum, plasma (heparin) and cell culture supernatants. |
| Standard Range | 15.6 - 1000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5% Inter-Assay CV: 6.3% |
| Alternate Names | MDC9; Meltrin-gamma; ADAM 9; Metalloprotease/disintegrin/cysteine-rich protein 9; Disintegrin and metalloproteinase domain-containing protein 9; Cellular disintegrin-related protein; EC 3.4.24.-; CORD9; Mltng; MCMP; Myeloma cell metalloproteinase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13443 Human Disintegrin and metalloproteinase domain-containing protein 9 |
|---|---|
| Gene Symbol | ADAM9 |
| Gene Full Name | ADAM metallopeptidase domain 9 |
| Background | This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010] |
| Function | Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. [UniProt] |
| Highlight | Related products: ADAM antibodies; ADAM ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | Proteolytically cleaved in the trans-Golgi network before it reaches the plasma membrane to generate a mature protein. The removal of the pro-domain occurs via cleavage at two different sites. Processed most likely by a pro-protein convertase such as furin, at the boundary between the pro-domain and the catalytic domain. An additional upstream cleavage pro-protein convertase site (Arg-56/Glu-57) has an important role in the activation of ADAM9. Phosphorylation is induced in vitro by phorbol-12-myristate-13-acetate (PMA). [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81558 Human ADAM9 ELISA Kit standard curve image
ARG81558 Human ADAM9 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81558 Human ADAM9 ELISA Kit User's manual |
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