ARG81933
Human ADAM15 ELISA Kit
Human ADAM15 ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81933-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81933-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81933-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81933-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81933-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81933-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81933-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81933-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81933-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81933-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81933-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81933 Human ADAM15 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADAM15 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ADAM15 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 31.25 pg/ml |
| Sample Type | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| Standard Range | 62.5 - 4000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.5%; Inter-Assay CV: 6.6% |
| Alternate Names | ADAM 15; MDC-15; EC 3.4.24.-; Metargidin; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; Metalloprotease RGD disintegrin protein; MDC15; Disintegrin and metalloproteinase domain-containing protein 15 |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13444 Human Disintegrin and metalloproteinase domain-containing protein 15 |
|---|---|
| Gene Symbol | ADAM15 |
| Gene Full Name | ADAM metallopeptidase domain 15 |
| Background | The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Function | Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. [UniProt] |
| Highlight | Related products: ADAM antibodies; ADAM ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | The precursor is cleaved by a furin endopeptidase. Phosphorylation increases association with PTKs. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81933 Human ADAM15 ELISA Kit standard curve image
ARG81933 Human ADAM15 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81933 Human ADAM15 ELISA Kit User's manual |
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