ARG81928
Human ADA ELISA Kit
Human ADA ELISA Kit for ELISA and Human
Component
| Cat No | Component Name | Package | Temp |
|---|---|---|---|
| ARG81928-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81928-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81928-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81928-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81928-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81928-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81928-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81928-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81928-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81928-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81928-011 | Plate sealer | 4 strips | Room temperature |
Overview
| Product Description | ARG81928 Human ADA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ADA in serum, pleural fluid and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ADA |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 15.6 pg/ml |
| Sample Type | Serum, pleural fluid and cell culture supernatants. |
| Standard Range | 31.2 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 5.0%; Inter-Assay CV: 5.7% |
| Alternate Names | Adenosine deaminase; EC 3.5.4.4; Adenosine aminohydrolase |
Application Instructions
| Assay Time | ~ 5 hours |
|---|
Properties
| Form | 96 well |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | ADA |
| Gene Full Name | adenosine deaminase |
| Background | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. [UniProt] |
| Highlight | Related products: ADA antibodies; ADA ELISA Kits; ADA Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG81928 Human ADA ELISA Kit standard curve image
ARG81928 Human ADA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81928 Human ADA ELISA kit User manual |
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