ARG83225
Human ACY1 / Aminoacylase 1 ELISA Kit
Human ACY1 / Aminoacylase 1 ELISA Kit for ELISA and Human
Overview
| Product Description | ARG83225 Human ACY1 / Aminoacylase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ACY1 / Aminoacylase 1 in Serum, Plasma and Cell culture supernatants. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Specificity | There is no detectable cross-reactivity with other relevant proteins. |
| Target Name | ACY1 / Aminoacylase 1 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 25 pg/ml |
| Detection Range | 312 pg/ml - 20,000 pg/ml |
| Sample Type | Serum, Plasma and Cell culture supernatants |
| Precision | Intra-Assay CV: 5.0% Inter-Assay CV: 4.6% |
| Alternate Names | ACY-1; N-acyl-L-amino-acid amidohydrolase; ACY1D; EC 3.5.1.14; HEL-S-5; Aminoacylase-1 |
Application Instructions
| Assay Time | ~ 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | ACY1 |
| Gene Full Name | aminoacylase 1 |
| Background | This gene encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. This gene is located on chromosome 3p21.1, a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in this gene cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID:100526760. A related pseudogene has been identified on chromosome 18. [provided by RefSeq, Nov 2010] |
| Function | Involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG83225 Human ACY1 / Aminoacylase 1 ELISA Kit standard curve image
ARG83225 Human ACY1 / Aminoacylase 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83225 Human ACY1 / Aminoacylase 1 ELISA Kit User manual |
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