ARG80856
Human 17-OH Progesterone (free) ELISA Kit
Human 17-OH Progesterone (free) ELISA Kit for ELISA and Human
Signaling Transduction kit
Overview
| Product Description | ARG80856 Human 17-OH Progesterone (free) ELISA Kit is an enzyme immunoassay kit for the quantification of active free 17-hydroxyprogesterone in saliva. Measurements of 17-hydroxyprogesterone are used as an aid in the research of various disorders of the adrenal glands or the ovaries, and as an aid in the diagnosis of late onset of 21-hydroxylase deficiency, a common cause of Congenital Adrenal Hyperplasia. This test is not intended for newborn screening. |
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| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Target Name | 17-OH Progesterone |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 2.5 pg/ml |
| Sample Type | Saliva. |
| Standard Range | 10 - 1000 pg/ml |
| Sample Volume | 25 µl |
Application Instructions
| Assay Time | 60, 15 min |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | 17-OH-Progesterone |
|---|---|
| Background | 17 alpha-Hydroxyprogesterone (17OHP) is a 21-carbon steroid produced by adrenals, ovaries, testes and placenta. 17OHP is the metabolite of progesterone and 17-hydroxyprognenolone, and is a precursor to cortisol. Serum levels are primarily used to diagnose congenital adrenal hyperplasia (CAH). Deficiency of 21-hydroxylase blocks synthesis of cortisol, resulting in an increase of serum 17OHP levels, which leads to increase in ACTH secretion. Besides the classical CAH, late-onset and milder heterozygous forms of CAH also occur. They may clinically manifest themselves during the peripubertal period in the form of menstrual disturbances and hirsutism. Even in adulthood, hirsutism can signal the possible presence of a heterozygous form of CAH. Measurements of basal and post-ACTH stimulation 17OHP levels are helpful in the differential diagnosis of these conditions. The measurement of 17OHP in serum or plasma samples from babies or young children is difficult because the high concentrations of conjugates present in blood. Therefore such serum and plasma samples should be extracted prior to the assay. The importance of the extraction step increases with decreasing age of the patient. In saliva the interference with the conjugates is not relevant at all, as conjugates are not present in saliva. Therefore salivary measurements of 17OHP even in newborns can be done without extraction step! This is not possible in serum or plasma samples. |
| Highlight | Related products: 17-OH-Progesterone ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| Research Area | Signaling Transduction kit |
Images (1) Click the Picture to Zoom In
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ARG80856 Human 17-OH Progesterone (free) ELISA Kit example of standard curve image
ARG80856 Human 17-OH Progesterone (free) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80856 Human 17-OH Progesterone (free) ELISA Kit User's manual |
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