ARG81149
HSP70 (High sensitive) ELISA Kit
HSP70 (High sensitive) ELISA Kit for ELISA and Human,Mouse,Rat,Dog,Monkey
Overview
| Product Description | ARG81149 HSP70 (High sensitive) ELISA Kit is a high sensitive Enzyme Immunoassay kit for the quantification of HSP70 in cell/tissue lysates, plasma and serum. |
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| Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
| Tested Application | ELISA |
| Target Name | Hsp 70 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm |
| Sensitivity | 0.031 ng/ml |
| Sample Type | Cell/tissue lysates, plasma and serum. |
| Standard Range | 0.42 - 27 ng/ml |
| Sample Volume | 100 µl |
| Alternate Names | Heat shock 70 kDa protein 1A; HSPA1; HSP70I; Heat shock 70 kDa protein 1; HSP70-1A; HEL-S-103; HSP70.1; HSP72; HSP70-1 |
Application Instructions
| Assay Time | 5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HSPA1A |
| Gene Full Name | heat shock 70kDa protein 1A |
| Background | This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008] |
| Function | In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. [UniProt] |
| Highlight | Related products: Hsp70 antibodies; Hsp70 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress. |
Images (1) Click the Picture to Zoom In
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ARG81149 HSP70 (High sensitive) ELISA Kit standard curve image
ARG81149 HSP70 (High sensitive) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG81149 HSP70 (High sensitive) ELISA Kit User's manual |
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