ARG82813
Guinea Pig IL8 ELISA Kit
Guinea Pig IL8 ELISA Kit for ELISA and Guinea pig
Overview
| Product Description | ARG82813 Guinea Pig IL8 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Guinea Pig IL8 in serum, plasma and cell culture supernatants. |
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| Tested Reactivity | Gpig |
| Tested Application | ELISA |
| Specificity | Cross-Reactivity: Not react with BMP1, HGF, IL1 beta, IFN gamma, MMP2, TGF beta 1, TLR1, TNF alpha and VEGF. |
| Target Name | IL8 |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 6.3 pg/ml |
| Sample Type | Serum, plasma and cell culture supernatants. |
| Standard Range | 12.5 - 800 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6.0% Inter-Assay CV: 9.0% |
| Alternate Names | IL8/NAP1 form IV; GCP/IL-8 protein IV; NAF; T-cell chemotactic factor; 1-77; Ala-IL-8; Interleukin-8; IL-8; Neutrophil-activating protein 1; GCP/IL-8 protein II; IL8/NAP1 form II; GCP/IL-8 protein V; MDNCF; Protein 3-10C; Lymphocyte-derived neutrophil-activating factor; Neutrophil-activating factor; Granulocyte chemotactic protein 1; LYNAP; NAP-1; Monocyte-derived neutrophil chemotactic factor; 6-77; 7-77; C-X-C motif chemokine 8; GCP1; NAP1; Ser-IL-8; 5-77; GCP/IL-8 protein VI; IL8/NAP1 form I; IL8/NAP1 form VI; Monocyte-derived neutrophil-activating peptide; C-X-C motif; 8-77; 9-77; LUCT; Chemokine; GCP-1; MDNCF-b; MDNCF-c; IL8/NAP1 form V; LECT; IL8/NAP1 form III; GCP/IL-8 protein III; Emoctakin; GCP/IL-8 protein I; MONAP; IL8 |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CXCL8 |
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| Gene Full Name | chemokine (C-X-C motif) ligand 8 |
| Background | The protein encoded by this gene is a member of the CXC chemokine family and is a major mediator of the inflammatory response. The encoded protein is secreted primarily by neutrophils, where it serves as a chemotactic factor by guiding the neutrophils to the site of infection. This chemokine is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other members of the CXC chemokine gene family form a gene cluster in a region of chromosome 4q. [provided by RefSeq, Aug 2017] |
| Function | IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| PTM | Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form. Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82813 Guinea Pig IL8 ELISA Kit standard curve image
ARG82813 Guinea Pig IL8 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82813 Guinea Pig IL8 ELISA Kit User manual |
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