ARG21689

Goat anti-Rat IgG antibody (TRITC), pre-adsorbed

Goat anti-Rat IgG antibody (TRITC), pre-adsorbed for Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,Western blot and Rat

publication_link Publication2

Overview

Product Description TRITC-conjugated Goat Polyclonal antibody recognizes Rat IgG
Tested Reactivity Rat
Tested Application FACS, FLISA, ICC/IF, IHC-Fr, WB
Specificity The antibody reacts with the heavy chain of Rat IgG. The antibody is pre-adsorbed with Rat IgM, so the antibody may not react with Rat IgM, but may react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG
Antigen Species Rat
Conjugation TRITC

Application Instructions

Pre Adsorbed Rat IgM.
Application Suggestion
Tested Application Dilution
FACSAssay-dependent
FLISA1:100 - 1:400
ICC/IFAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21689 Goat anti-Rat IgG antibody (TRITC), pre-adsorbed IHC-Fr image

    Immunohistochemistry: Mouse tissue stained with ARG20565 anti-CD45 antibody [I3/2.3] at 1:100 dilution.
    ARG21689 Goat anti-Rat IgG antibody (TRITC), pre-adsorbed used for secondary antibody at 1:400 dilution.

    From Wai-Sam Ao-Ieong et al. Polymers (Basel). (2021), doi: 10.3390/polym13121960, Fig. 7A, B.

  • ARG21689 Goat anti-Rat IgG antibody (TRITC) (pre-adsorbed) FLISA image

    FLISA: The plate was coated with purified Rat IgG and IgM. Immunoglobulins were detected with serially diluted ARG21689 Goat anti-Rat IgG antibody (TRITC) (pre-adsorbed).

Specific References

The Effect of Heat Treatment toward Glycerol-Based, Photocurable Polymeric Scaffold: Mechanical, Degradation and Biocompatibility.

IHC-Fr / Rat

Wai-Sam Ao-Ieong et al.
Polymers (Basel).,  (2021)

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The selection of photoinitiators for photopolymerization of biodegradable polymers and its application in digital light processing additive manufacturing

IHC-Fr / 

Chia-Chun Wang et al.
J Biomed Mater Res A.,  (2021)

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