ARG23770

Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed

Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed for ELISA,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Rat IgG (H+L)
Tested Reactivity Rat
Tested Application ELISA, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Reacts with the heavy and light chains of rat IgG and with the light chains of rat IgM as demonstrated by ELISA, FLISA, and/or flow cytometry; minimal cross reactivity with mouse immunoglobulins
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG (H+L)
Antigen Species Rat
Conjugation Biotin

Application Instructions

Pre Adsorbed Mouse immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
FACS< 1 µg/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (4) Click the Picture to Zoom In

  • ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed FACS image

    Flow Cytometry: C57BL/6 Mouse splenocytes were stained with ARG20565 anti-CD45 antibody [I3/2.3] followed by ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed followed by Streptavidin-FITC.

  • ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed IHC-Fr image

    Immunohistochemistry: Frozen section of BALB/c Mouse intestine was stained with ARG21041 anti-Integrin beta 4 antibody [346-11A] (right) followed by ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed, and Streptavidin-FITC.

  • ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse adductor muscle day 3 post femoral artery ligation was stained with ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed and then Streptavidin-HRP, hematoxylin, and AEC.

  • ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin), pre-adsorbed IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse adductor muscle day 7 post femoral artery ligation was stained with anti-Mac-3 followed by ARG23770 Goat anti-Rat IgG (H+L) antibody (Biotin) (pre-adsorbed) and then ARG23912 Streptavidin (HRP), hematoxylin, and AEC.