ARG21917

Goat anti-Rabbit IgG antibody (TRITC), pre-adsorbed

Goat anti-Rabbit IgG antibody (TRITC), pre-adsorbed for Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rabbit

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Overview

Product Description TRITC-conjugated Goat Polyclonal antibody recognizes Rabbit IgG
Tested Reactivity Rb
Tested Application FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with the heavy chain of Rabbit IgG. The antibody is pre-adsorbed with Rabbit IgM, so the antibody may not react with Rabbit IgM, but may react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG
Antigen Species Rabbit
Conjugation TRITC

Application Instructions

Pre Adsorbed Rabbit IgM.
Application Suggestion
Tested Application Dilution
FACSAssay-dependent
FLISA1:100 - 1:400
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (1) Click the Picture to Zoom In

  • ARG21917 Goat anti-Rabbit IgG antibody (TRITC) (pre-adsorbed) FLISA image

    FLISA: The plate was coated with purified rabbit IgG and IgM. Immunoglobulins were detected with serially diluted ARG21917 Goat anti-Rabbit IgG antibody (TRITC) (pre-adsorbed).

Customer's Feedback

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Review for Goat anti-Rabbit IgG antibody (TRITC), pre-adsorbed

Application:IF/ICC

Sample:BV-2

Fixation Buffer:4% Paraformaldehyde

Fixation Time:20 min

Fixation Temperature:Room Temperature ºC

Primary Antibody Dilution Factor:1 : 100

Primary Antibody Incubation Time:Overnight

Primary Antibody Incubation Temperature:4 ºC