ARG21894
Goat anti-Mouse IgG2b antibody (HRP), pre-adsorbed
Goat anti-Mouse IgG2b antibody (HRP), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse
Overview
| Product Description | HRP-conjugated Goat Polyclonal antibody recognizes Mouse IgG2b |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Reacts with the heavy chain of mouse IgG2b |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IgG2b |
| Antigen Species | Mouse |
| Conjugation | HRP |
Application Instructions
| Pre Adsorbed | Mouse IgG1, IgG2a, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera. | ||||||||||||||||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 50% PBS (pH 7.4) and 50% Glycerol |
| Stabilizer | 50% Glycerol |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (7) Click the Picture to Zoom In
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image
Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Histone H3 acetyl (Lys4) antibody followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) WB image
Western blot: Jurkat whole cell lysate untreated (left) or TSA-treated (400 nM, 12 hours) (right). The blots were stained with anti-Histone H3 acetyl (Lys4) antibody. Secondary antibody: ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed).
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ELISA image
ELISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed).
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image
Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-CD44 antibody and anti-Histone H3 acetyl (Lys4) antibody, followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP) (pre-adsorbed) and ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), Red AP, DAB and mounted with Fluoromount-G.
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image
Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Histone H3 phospho (Thr3) antibody (right), followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image
Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with ARG21506 anti-MMP1 antibody [SB12e] followed by ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), DAB and mounted with Fluoromount-G.
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ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed) ICC image
Immunocytochemistry: Human pancreatic carcinoma cell line MIA PaCa-2 stained with ARG22307 anti-Cytokeratin 8 antibody [SB37b] and ARG21506 anti-MMP1 antibody [SB12e], followed by ARG23804 Goat anti-Mouse IgG2a antibody (AP) (pre-adsorbed), ARG21894 Goat anti-Mouse IgG2b antibody (HRP) (pre-adsorbed), Red AP, DAB and mounted with Fluoromount-G.
