ARG21892

Goat anti-Mouse IgG2b antibody (FITC), pre-adsorbed

Goat anti-Mouse IgG2b antibody (FITC), pre-adsorbed for Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description FITC-conjugated Goat Polyclonal antibody recognizes Mouse IgG2b
Tested Reactivity Ms
Tested Application FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Reacts with the heavy chain of mouse IgG2b
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG2b
Antigen Species Mouse
Conjugation FITC

Application Instructions

Pre Adsorbed Mouse IgG1, IgG2a, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
FACS< 1 ug/10^6 cells
FLISA1:100 - 1:400
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21892 Goat anti-Mouse IgG2b antibody (FITC) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 stained with anti-Cytokeratin 18 antibody (right) followed by ARG21892 Goat anti-Mouse IgG2b antibody (FITC) (pre-adsorbed) and DAPI.

  • ARG21892 Goat anti-Mouse IgG2b antibody (FITC) (pre-adsorbed) FLISA image

    FLISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM and IgA. Immunoglobulins were detected with serially diluted ARG21892 Goat anti-Mouse IgG2b antibody (FITC) (pre-adsorbed).