ARG21898

Goat anti-Mouse IgG2b antibody, F(ab')2 fragment (Biotin), pre-adsorbed

Goat anti-Mouse IgG2b antibody, F(ab')2 fragment (Biotin), pre-adsorbed for ELISA,Flow cytometry,FLISA,ICC/IF and Mouse

Overview

Product Description Biotin-conjugated F(ab')2 fragment of Goat Polyclonal antibody recognizes Mouse IgG2b
Tested Reactivity Ms
Tested Application ELISA, FACS, FLISA, ICC/IF
Specificity Reacts with the heavy chain of mouse IgG2b
Host Goat
Clonality Polyclonal
Isotype F(ab')2 IgG
Target Name IgG2b
Antigen Species Mouse
Conjugation Biotin

Application Instructions

Pre Adsorbed Mouse IgG1, IgG2a, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:10000
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21898 Goat anti-Mouse IgG2b antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 stained with anti-Cytokeratin 18 antibody (right) and ARG22146 anti-CD44 antibody [F10-44-2] (FITC) followed by ARG21898 Goat anti-Mouse IgG2b antibody, F(ab')2 fragment (Biotin) (pre-adsorbed), Streptavidin (Cy3) and DAPI.

  • ARG21898 Goat anti-Mouse IgG2b antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG21898 Goat anti-Mouse IgG2b antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).