ARG23801

Goat anti-Mouse IgG2a antibody, pre-adsorbed

Goat anti-Mouse IgG2a antibody, pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Purification,Western blot and Mouse

Overview

Product Description Goat Polyclonal antibody recognizes Mouse IgG2a
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG2a
Antigen Species Mouse
Conjugation Un-conjugated

Application Instructions

Pre Adsorbed Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ELISPOTAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2).
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG23801 Goat anti-Mouse IgG2a antibody (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA and Human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23801 Goat anti-Mouse IgG2a antibody (pre-adsorbed) followed by Mouse anti-Goat IgG (Fc) antibody (HRP).

  • ARG23801 Goat anti-Mouse IgG2a antibody, pre-adsorbed FLISA image

    FLISA: The plate was coated with ARG21883 Goat anti-Mouse IgG1 antibody (pre-adsorbed), ARG23801 Goat anti-Mouse IgG2a antibody (pre-adsorbed), ARG21891 Goat anti-Mouse IgG2b antibody (pre-adsorbed), Goat anti-Mouse IgG3 antibody, ARG21539 Goat anti-Mouse IgA antibody (pre-adsorbed), and ARG21517 Goat anti-Mouse IgM antibody (pre-adsorbed). Serially diluted ARG23894 Mouse IgG2a Lambda Isotype Control antibody [HOPC-1] (FITC) was captured and fluorescence intensity quantified.