ARG23806
Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed
Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Purification,Western blot and Mouse
Overview
| Product Description | Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB |
| Specificity | Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IgG2a |
| Antigen Species | Mouse |
| Conjugation | Biotin |
Application Instructions
| Pre Adsorbed | Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera. | ||||||||||||||||||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.5 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (4) Click the Picture to Zoom In
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ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ICC/IF image
Immunofluorescence: TGFB1 stimulated Human dermal fibroblasts were stained with anti-ACTA2 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed, Cy3 conjugated Streptavidin, and DAPI.
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ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed FACS image
Flow Cytometry: Human blood peripheral lymphocytes were stained with Mouse anti-Human CD5 followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-FITC.
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ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed IHC image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue was stained with ARG22191 anti-MMP9 antibody [SB15c] followed by ARG23806 Goat anti-Mouse IgG2a antibody (Biotin) (pre-adsorbed), ARG23912 Streptavidin (HRP), DAB, and hematoxylin.
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ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed ELISA image
ELISA: ELISA plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23806 Goat anti-Mouse IgG2a antibody (Biotin), pre-adsorbed followed by Streptavidin-HRP.
