ARG23809

Goat anti-Mouse IgG2a antibody (APC), pre-adsorbed

Goat anti-Mouse IgG2a antibody (APC), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Purification,Western blot and Mouse

Overview

Product Description APC-conjugated Goat Polyclonal antibody recognizes Mouse IgG2a
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, Puri, WB
Specificity Reacts with the heavy chain of mouse IgG2a as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with human immunoglobulins. May react with immunoglobulins from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG2a
Antigen Species Mouse
Conjugation APC

Application Instructions

Pre Adsorbed Mouse IgG1, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ELISPOTAssay-dependent
FACS< 0.1 µg/10^6 cells
FLISA< 1 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
PuriAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG23809 Goat anti-Mouse IgG2a antibody (APC), pre-adsorbed FACS image

    Flow Cytometry: Human blood peripheral lymphocytes were stained with Mouse anti-Human CD5 followed by ARG23809 Goat anti-Mouse IgG2a antibody (APC), pre-adsorbed.

  • ARG23809 Goat anti-Mouse IgG2a antibody (APC), pre-adsorbed FLISA image

    FLISA: FLISA plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23809 Goat anti-Mouse IgG2a antibody (APC), pre-adsorbed.