ARG21887

Goat anti-Mouse IgG1 antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgG1 antibody (Biotin), pre-adsorbed for ELISA,ELISPOT,Electron microscopy,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG1
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, EM, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Reacts with the heavy chain of mouse IgG1
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG1
Antigen Species Mouse
Conjugation Biotin

Application Instructions

Pre Adsorbed Mouse IgG2a, IgG2b, IgG3, IgM, and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
EMAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21887 Goat anti-Mouse IgG1 antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG1, IgG2a, IgG2b, IgG3, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG21887 Goat anti-Mouse IgG1 antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG21887 Goat anti-Mouse IgG1 antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with Mouse anti-Human CD3 antibody followed by ARG21887 Goat anti-Mouse IgG1 antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).