ARG23487

Goat anti-Mouse IgG antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgG antibody (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

publication_link Publication1

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Reacts with the heavy chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG
Antigen Species Mouse
Immunogen Mouse IgG
Target Ig IgG
Conjugation Biotin

Application Instructions

Pre Adsorbed Mouse IgM and IgA; Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 µg/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:100 - 1:500
IHC-P1:250 - 1:1500
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 stained with anti-Human CD44 antibody (right), followed by ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with Mouse anti-Human CD3 antibody followed by ARG23487 Goat anti-Mouse IgG antibody (Biotin) (pre-adsorbed) followed by Streptavidin (FITC).

Specific References

Assembly of Interfacial Polyelectrolyte Complexation Fiber Bundles with Mineralization Gradient for Physiologically-inspired Ligament Regeneration

IHC-P / 

Tzu-Wei Wang et al.
,  (2023)

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