ARG23799

Goat anti-Mouse IgG (H+L) antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgG (H+L) antibody (Biotin), pre-adsorbed for ELISA,Flow cytometry,FLISA,ICC/IF,Western blot and Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG (H+L)
Tested Reactivity Ms
Tested Application ELISA, FACS, FLISA, ICC/IF, WB
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, and with the light chains of mouse IgM and IgA as demonstrated by ELISA, FLISA, and/or flow cytometry. Minimal cross reactivity with rat immunoglobulins.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG (H+L)
Antigen Species Mouse
Conjugation Biotin

Application Instructions

Pre Adsorbed Rat immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
FACS< 1 µg/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG23799 Goat anti-Mouse IgG (H+L) antibody (Biotin) (pre-adsorbed) ICC/IF image

    Immunofluorescence: Human pancreatic carcinoma cell line MIA PaCa-2 stained with anti-Cytokeratin 18 antibody (right), followed by Goat anti-Mouse IgG (H+L) antibody, ARG23799 Goat anti-Mouse IgG (H+L) antibody (Biotin) (pre-adsorbed), Streptavidin (FITC) and DAPI.

  • ARG23799 Goat anti-Mouse IgG (H+L) antibody (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with Mouse anti-Human CD3 antibody followed by Goat anti-Mouse IgG (H+L) antibody, ARG23799 Goat anti-Mouse IgG (H+L) antibody (Biotin) (pre-adsorbed) and Streptavidin (FITC).