ARG23486

Goat anti-Mouse IgG (H+L) antibody (Biotin), pre-adsorbed

Goat anti-Mouse IgG (H+L) antibody (Biotin), pre-adsorbed for ELISA,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Mouse IgG (H+L)
Tested Reactivity Ms
Tested Application ELISA, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, IgG2c, and IgG3 and with the light chains of mouse IgM and IgA.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgG (H+L)
Antigen Species Mouse
Immunogen Mouse IgG
Target Ig IgG
Conjugation Biotin

Application Instructions

Pre Adsorbed Human immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
FACSAssay-dependent
FLISA< 1 µg/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG23486 Goat anti-Mouse IgG (H+L) antibody (Biotin) pre-adsorbed ICC/IF image

    Immunofluorescence: Human epithelial carcinoma cell line HEp-2 was stained with Mouse anti-Human CD44 followed by ARG23486 Goat anti-Mouse IgG (H+L) antibody (Biotin) pre-adsorbed, Streptavidin-FITC and DAPI.

  • ARG23486 Goat anti-Mouse IgG (H+L) antibody (Biotin) pre-adsorbed FACS image

    Flow Cytometry: Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3-UNLB followed by ARG23486 Goat anti-Mouse IgG (H+L) antibody (Biotin) pre-adsorbed followed by Streptavidin-FITC.