ARG23710
Goat anti-Mouse IgA + IgG + IgM antibody (HRP), pre-adsorbed
Goat anti-Mouse IgA + IgG + IgM antibody (HRP), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse
Overview
| Product Description | HRP-conjugated Goat Polyclonal antibody recognizes Mouse IgA + IgG + IgM |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, ELISPOT, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | Reacts with the heavy and light chains of Mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA, FLISA and flow cytometry. Minimal cross reactivity with human immunoglobulins. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IgA + IgG + IgM |
| Antigen Species | Mouse |
| Immunogen | Mouse IgG, IgM, and IgA. |
| Conjugation | HRP |
Application Instructions
| Pre Adsorbed | Human immunoglobulins and pooled sera. | ||||||||||||||||||
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| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography on mouse IgG, IgM, and IgA covalently linked to agarose. |
| Buffer | PBS (pH 7.4) and 50% Glycerol. |
| Stabilizer | 50% Glycerol |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (2) Click the Picture to Zoom In
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ARG23710 Goat anti-Mouse IgA + IgG + IgM antibody (HRP) (pre-adsorbed) WB image
Western blot: H4 human neuroglioma cell line coexpressing human αsyn tagged with either the amino-terminal (SL1) or the carboxyterminal fragments (SL2) of Gaussia princeps luciferase (SL1SL2 cells) were treated with tetracycline to block the expression of SL1 and SL2 and SL1SL2 exosomes were resolved by electrophoresis, transferred to PVDF membrane, and stained with anti-CD9 followed by ARG23710 Goat anti-Mouse IgA + IgG + IgM antibody (HRP) (pre-adsorbed) and chemiluminescent detection.
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ARG23710 Goat anti-Mouse IgA + IgG + IgM antibody (HRP) (pre-adsorbed) WB image
Western blot: Supernatant fractions from Bax-/- mouse embryonic fibroblasts were incubated with caspase-8-cleaved human Bid (tBid) and anti-Bak (Clone 7D10 and Clone 8F8), resolved by electrophoresis, transferred to membrane, and stained with anti-cytochrome c followed by ARG23710 Goat anti-Mouse IgA + IgG + IgM antibody (HRP) (pre-adsorbed) and chemiluminescent detection.
