ARG24085

Goat anti-Human Kappa Light Chain antibody, pre-adsorbed

Goat anti-Human Kappa Light Chain antibody, pre-adsorbed for ELISA,Flow cytometry,Western blot and Human

Overview

Product Description Goat Polyclonal antibody recognizes Human Kappa Light Chain
Tested Reactivity Hu
Tested Application ELISA, FACS, WB
Specificity The antibody reacts with human kappa light chains. The antibody is pre-adsorbed with human lambda light chains, mouse immunoglobulins and pooled sera. The antibody may react with kappa light chains from other species.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Kappa Light Chain
Antigen Species Human
Immunogen Human Kappa Light Chain.
Conjugation Un-conjugated
Alternate Names Ig kappa chain

Application Instructions

Pre Adsorbed Human lambda light chains, mouse immunoglobulins and pooled sera.
Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 µg/10^6 cells
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer BBS (pH 8.2)
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG24085 Goat anti-Human Kappa Light Chain antibody (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ and mouse IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted ARG24085 Goat anti-Human Kappa Light Chain antibody (pre-adsorbed), followed by Mouse anti-Goat IgG Fc antibody (HRP).

  • ARG24085 Goat anti-Human Kappa Light Chain antibody (pre-adsorbed) FACS image

    Flow Cytometry: CD19+ human B-lymphocytes were stained with ARG24085 Goat anti-Human Kappa Light Chain antibody (pre-adsorbed) and Goat F(ab')2 anti-Human Lambda Light Chain antibody (PE), followed by Mouse anti-Goat IgG Fc antibody (Alexa Fluor® 488).