ARG21680

Goat anti-Human Kappa Light Chain antibody, F(ab')2 fragment (Biotin), pre-adsorbed

Goat anti-Human Kappa Light Chain antibody, F(ab')2 fragment (Biotin), pre-adsorbed for ELISA,ELISPOT,Flow cytometry,FLISA,ICC/IF,Western blot and Human

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Human Kappa Light Chain
Tested Reactivity Hu
Tested Application ELISA, ELISPOT, FACS, FLISA, ICC/IF, WB
Specificity The antibody reacts with Human kappa light chains. The antibody is pre-adsorbed with Human lambda light chains, so the antibody may not react with Human lambda light chains, but may react with kappa light chains from other species.
Host Goat
Clonality Polyclonal
Isotype F(ab')2 IgG
Target Name Kappa Light Chain
Antigen Species Human
Conjugation Biotin

Application Instructions

Pre Adsorbed Human lambda light chains.
Application Suggestion
Tested Application Dilution
ELISA1:5000 - 1:20000
ELISPOTAssay-dependent
FACS< 1 ug/10^6 cells
FLISAAssay-dependent
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (2) Click the Picture to Zoom In

  • ARG21680 Goat anti-Human Kappa Light Chain antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) ELISA image

    ELISA: The plate was coated with purified Human IgGκ, IgMκ, IgAκ, IgGλ, IgMλ, and IgAλ. Immunoglobulins were detected with serially diluted ARG21680 Goat anti-Human Kappa Light Chain antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) followed by ARG23912 Streptavidin (HRP).

  • ARG21680 Goat anti-Human Kappa Light Chain antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) FACS image

    Flow Cytometry: CD19+ Human B-lymphocytes stained with ARG21680 Goat anti-Human Kappa Light Chain antibody, F(ab')2 fragment (Biotin) (pre-adsorbed) and Goat F(ab')2 anti-Human Lambda Light Chain antibody (PE) followed by Streptavidin (FITC).