ARG24032

Goat anti-Human IgE antibody (Biotin)

Goat anti-Human IgE antibody (Biotin) for ELISA,Flow cytometry,Western blot and Human,Rhesus Monkey

Overview

Product Description Biotin-conjugated Goat Polyclonal antibody recognizes Human IgE
Tested Reactivity Hu, R. Mk
Tested Application ELISA, FACS, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IgE
Antigen Species Human
Immunogen Native Human IgE.
Conjugation Biotin

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:10000 - 1:100000
FACSAssay-dependent
WB1:10000 - 1:100000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified.
Buffer PBS, 0.09% Sodium azide and 0.2% BSA.
Preservative 0.09% Sodium azide
Stabilizer 0.2% BSA
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (3) Click the Picture to Zoom In

  • ARG24032 Goat anti-Human IgE antibody (Biotin) standard curve image

    ELISA: Mouse anti-Human IgE antibody as a capture antibody at 5 µg/ml dilution and ARG24032 Goat anti-Human IgE antibody (Biotin) as a detection antibody at 2 µg/ml dilution with Purified Human IgE as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.

  • ARG24032 Goat anti-Human IgE antibody (Biotin) standard curve image

    ELISA: Mouse anti-Human IgE antibody as a capture antibody at 5 µg/ml dilution and ARG24032 Goat anti-Human IgE antibody (Biotin) as a detection antibody at 2 µg/ml dilution with Recombinant Human IgE Lambda as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.

  • ARG24032 Goat anti-Human IgE antibody (Biotin) standard curve image

    ELISA: Mouse anti-Human IgE antibody as a capture antibody at 5 µg/ml dilution and ARG24032 Goat anti-Human IgE antibody (Biotin) as a detection antibody at 2 µg/ml dilution with Recombinant Human IgE Kappa as antigen for generation of the standard curve. Detection is by HRP conjugated streptavidin and substrate. Plasma and serum samples diluted 1:5 are indicated in green and orange respectively.