ARG83368

Glycine Assay Kit

Glycine Assay Kit for Functional study and All species

Flavor enhancers, Glucogenic amino acids, Inhibitory amino acids, Proteinogenic amino acids, Glycine receptor agonists, NMDA receptor agonists, E-number additives

Overview

Product Description ARG83368 Glycine Assay Kit is an Assay kit for the quantification of Glycine in cell lysates, serum, plasma, saliva and urine.
Tested Reactivity All
Tested Application FuncSt
Target Name Glycine
Conjugation Un-conjugated
Conjugation Note Read at 530-570 nm.
Sensitivity 1.0 µM
Sample Type Cell lysates, Serum, Plasma, Saliva and Urine.
Standard Range 1.56 - 100 µM
Sample Volume 50 µL

Application Instructions

Assay Time ~1.5 hours

Properties

Form Liquid
Storage Instruction Store components at -20°C and -80°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Glycine is an amino acid that has a single hydrogen atom as its side chain. It is the simplest stable amino acid (carbamic acid is unstable), with the chemical formula NH2‐CH2‐COOH. Glycine is one of the proteinogenic amino acids. It is encoded by all the codons starting with GG (GGU, GGC, GGA, GGG). Glycine is integral to the formation of alpha-helices in secondary protein structure due to the "flexibility" caused by such a small R group. Glycine is also an inhibitory neurotransmitter – interference with its release within the spinal cord (such as during a Clostridium tetani infection) can cause spastic paralysis due to uninhibited muscle contraction.
Function The principal function of glycine is it acts as a precursor to proteins. Most proteins incorporate only small quantities of glycine, a notable exception being collagen, which contains about 35% glycine due to its periodically repeated role in the formation of collagen's helix structure in conjunction with hydroxyproline. In the genetic code, glycine is coded by all codons starting with GG, namely GGU, GGC, GGA and GGG.
Research Area Flavor enhancers, Glucogenic amino acids, Inhibitory amino acids, Proteinogenic amino acids, Glycine receptor agonists, NMDA receptor agonists, E-number additives

Images (1) Click the Picture to Zoom In

  • ARG83368 Glycine Assay Kit standard curve image

    ARG83368 Glycine Assay Kit results of a typical standard run with optical density reading at 450 nm.

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