ARG82117
Glutathione Peroxidase Assay Kit
Glutathione Peroxidase Assay Kit for Functional study and All species
Component
| Cat No | Component Name | Package |
|---|---|---|
| ARG82117-001 | Assay Buffer | 25 ml |
| ARG82117-002 | GR Enzyme | 250 μl |
| ARG82117-003 | Glutathione | 1 vial (lyophilized) |
| ARG82117-004 | NADPH | 1 vial (lyophilized) |
| ARG82117-005 | Standard | 1 vial (lyophilized) |
| ARG82117-006 | Peroxide Solution | 50 μl |
Overview
| Product Description | ARG82117 Glutathione Peroxidase Assay Kit is a detection kit for the quantification of Glutathione Peroxidase activity in serum, plasma, tissue and cell lysates. |
|---|---|
| Tested Reactivity | All |
| Tested Application | FuncSt |
| Target Name | Glutathione Peroxidase |
| Conjugation Note | Read at 340 nm. |
| Sensitivity | 40 U/l |
| Detection Range | 40 - 800 U/l |
| Sample Type | Serum, plasma, tissue and cell lysates. |
| Sample Volume | 10 µl |
| Alternate Names | GPXD; Glutathione peroxidase 1; GPx-1; Cellular glutathione peroxidase; EC 1.11.1.9; GSHPX1; GSHPx-1 |
Application Instructions
| Application Note | Note: Please note that this kit does not include a microplate. |
|---|---|
| Assay Time | 20 min |
Properties
| Form | Liquid |
|---|---|
| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | GPX1 |
|---|---|
| Gene Full Name | glutathione peroxidase 1 |
| Background | This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Protects the hemoglobin in erythrocytes from oxidative breakdown. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| PTM | During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82117 Glutathione Peroxidase Assay Kit standard curve image
ARG82117 Glutathione Peroxidase Assay Kit results of a typical standard run with optical density reading at 340 nm.
| Title | Download Link |
|---|---|
| ARG82117 Glutathione Peroxidase Assay Kit User manual |
 Download
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