ARG82165
Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric)
Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All species
Overview
| Product Description | ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) can be used to measure Glucose 6 phosphate Dehydrogenase activity in serum, plasma, tissue and cell culture supernatants. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | Glucose 6 phosphate Dehydrogenase |
| Conjugation Note | Read at 460 nm |
| Sensitivity | 0.2 U/L |
| Detection Range | 0.2 - 100 U/L |
| Sample Type | serum, plasma, tissue and cell culture supernatants |
| Sample Volume | 20 µl |
| Alternate Names | G6PD1; G6PD; EC 1.1.1.49; Glucose-6-phosphate 1-dehydrogenase |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 20 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05370 Rat Glucose-6-phosphate 1-dehydrogenase Swiss-port # P11413 Human Glucose-6-phosphate 1-dehydrogenase |
|---|---|
| Gene Symbol | G6PD |
| Gene Full Name | glucose-6-phosphate dehydrogenase |
| Background | This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. [UniProt] |
| PTM | Acetylated by ELP3 at Lys-403; acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403; deacetylation stimulates its enzyme activity. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) enzyme kinetics graph
Kinetics of 0 and 100 U/L G6PDH reaction, using ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric).
| Title | Download Link |
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| ARG82165 Glucose 6 phosphate Dehydrogenase Activity Assay Kit (Colorimetric) User's manual |
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