ARG65668
DefineBlot Mouse anti-Rabbit IgG antibody (HRP)
DefineBlot Mouse anti-Rabbit IgG antibody (HRP) for ELISA,Western blot and Rabbit
Immune System antibody
Overview
| Product Description | HRP-conjugated Mouse Monoclonal antibody recognizes native Rabbit IgG but not denatured Rabbit IgG |
|---|---|
| Tested Reactivity | Rb |
| Tested Application | ELISA, WB |
| Specificity | ARG65668 DefineBlot Mouse anti-Rabbit IgG antibody (HRP) only detecting native IgG but not denatured IgG is easy to replace traditional secondary antibodies and useful to decrease denatured IgG interference in Co-IP experiment. |
| Host | Mouse |
| Clonality | Monoclonal |
| Target Name | IgG |
| Antigen Species | Rabbit |
| Target Ig | Rabbit IgG |
| Conjugation | HRP |
Application Instructions
| Application Suggestion |
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| Application Note | In Co-IP experiment, denatured IgG from IP samples may interfere target signal in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. ARG65668 DefineBlot Mouse anti-Rabbit IgG antibody (HRP) only detecting native IgG but not denatured IgG is easy to replace traditional secondary antibodies and useful to decrease denatured IgG interference in Co-IP assay. WB: It is suggested using less than 10 μg of primary antibody for IP process, and we also recommend freshly adding 100-200 mM DTT in loading buffer for IP sample and boiling for 5-10 min to completely denature IgG. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.01% Thimerosal, 1% BSA, 10% Glycerol and other stabilizers |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 1% BSA, 10% Glycerol |
| Concentration | 0.4-0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Immune System antibody |
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