ARG70483
Clostridium difficile toxin B recombinant protein (His-tagged)
Clostridium difficile toxin B recombinant protein (His-tagged) for SDS-PAGE
Overview
| Product Description | E. coli expressed, His-tagged Clostridium difficile toxin B recombinant protein. |
|---|---|
| Tested Application | SDS-PAGE |
| Target Name | Clostridium difficile toxin B |
| Species | Others |
| A.A. Sequence | Met1-Leu543 |
| Expression System | E. coli |
| Protein Full Name | Toxin B |
Properties
| Form | Powder |
|---|---|
| Purification | >95% (by SDS-PAGE) |
| Purification Note | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| Buffer | PBS (pH 7.4) |
| Reconstitution | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| Storage Instruction | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Function | This activates the peptidase C80 domain and autocatalytic processing, releasing the N-terminal part (Glucosyltransferase TcdB), which constitutes the active part of the toxin, in the cytosol. [Uniprot] |
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