ARG81299

Cell Cycle / Apoptosis Analysis Kit

Cell Cycle / Apoptosis Analysis Kit for Functional study and Other

Component

Cat No Component Name Package
ARG81299-1 Staining Buffer 4 X 20 ml
ARG81299-2 25 X Propidium Iodide (PI) 2 X 1.5 ml
ARG81299-3 RNase A (2.5 mg/ml) 0.8 ml

Overview

Product Description ARG81299 Cell Cycle / Apoptosis Analysis Kit can be used to measure Cell Cycle / Apoptosis activity in cells.
Tested Reactivity Other
Tested Application FuncSt
Target Name Cell Cycle / Apoptosis
Conjugation Note Excitation: 488 nm.
Sample Type Cells
Sample Volume 50 µl

Application Instructions

Assay Time ~ 3 hours

Properties

Form Liquid
Storage Instruction Store components at 4°C or -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background The content of DNA is changed with the process of cell cycle. And the DNA can be stained by fluorescent dye to measure its intensity by flow cytometry to monitor the cell cycle distribution in G1, S, GS/M phase and as well as apoptosis and aneuploidy cells with signals at sub-G1 or super G2 region.
Propidium iodide (or PI) is a fluorescent intercalating agent and it binds to DNA by intercalating between the bases with little or no sequence preference. Therefore PI is used widely as a DNA dye in flow cytometry to evaluate cell viability or DNA content in cell cycle analysis, or in microscopy to visualize the nucleus and other DNA-containing organelles. However, PI also binds to RNA, and it interference with the DNA measurement in the assay. In this Cell Cycle / Apoptosis Analysis Kit we provide an RNase to degrade RNA before PI staining to eliminate the interference by RNA and the kit can be used to monitor cell cycle progression, proliferation and apoptosis by flow cytometry.

Images (1) Click the Picture to Zoom In

  • ARG81299 Cell Cycle / Apoptosis Analysis Kit functional study image

    Functional study: Jurkat cells stained with ARG81299 Cell Cycle / Apoptosis Analysis Kit.

Title Download Link
ARG81299 Cell Cycle / Apoptosis Analysis Kit User manual download icon Download