ARG83407
Caspase 3 Assay Kit
Caspase 3 Assay Kit for Functional study and Other
Overview
| Product Description | ARG83407 Caspase 3 Assay Kit can be used to measure Caspase 3 in tissue extracts, cell lysate and other biological fluids. |
|---|---|
| Tested Reactivity | Other |
| Tested Application | FuncSt |
| Target Name | Caspase 3 |
| Conjugation Note | Read at 405 nm |
| Sensitivity | 3 μmol/ml |
| Sample Type | Tissue extracts, cell lysate and other biological fluids. |
| Standard Range | 5 μmol/ml - 500 μmol/ml |
| Sample Volume | 40 μl |
| Alternate Names | CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama |
Application Instructions
| Assay Time | 1 hour |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 4°C and -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | CASP3 |
|---|---|
| Gene Full Name | caspase 3, apoptosis-related cysteine peptidase |
| Background | Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts. The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes that undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 and 7; and the protein itself is processed and activated by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein |
| Title | Download Link |
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| ARG83407 Caspase 3 Assay Kit User manual |
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