ARG82810
Caspase 1 Activity Assay Kit
Caspase 1 Activity Assay Kit for Functional study and Human,Mouse,Rat,All species
Overview
| Product Description | ARG82810 Caspase 1 Activity Assay Kit is a detection kit for the quantification of Caspase 1 activity in tissue extracts, cell lysates and other biological fluids. |
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| Tested Reactivity | Hu, Ms, Rat, All |
| Tested Application | FuncSt |
| Target Name | Caspase 1 |
| Conjugation Note | Read at 405 nm. |
| Detection Range | 5 - 500 µmol/L |
| Sample Type | Tissue extracts, cell lysates and other biological fluids. |
| Sample Volume | 40 µl |
| Alternate Names | Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 3.4.22.36 |
Application Instructions
| Assay Time | ~ 1 hour |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | CASP1 |
| Gene Full Name | caspase 1, apoptosis-related cysteine peptidase |
| Background | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012] |
| Function | Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Highlight | Related products: Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Related news: RIP1 activation and pathogenesis of NASH |
| PTM | The two subunits are derived from the precursor sequence by an autocatalytic mechanism. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82810 Caspase 1 Activity Assay Kit standard curve image
ARG82810 Caspase 1 Activity Assay Kit results of a typical standard run with optical density reading at 405 nm.
| Title | Download Link |
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| ARG82810 Caspase 1 Activity Assay Kit User manual |
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