ARG82643
Canine IL6 ELISA Kit
Canine IL6 ELISA Kit for ELISA and Dog
Overview
| Product Description | ARG82643 Canine IL6 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Canine IL6 in serum, plasma, cell lysate and tissue lysate. |
|---|---|
| Tested Reactivity | Dog |
| Tested Application | ELISA |
| Specificity | The following recombinant canine proteins were tested and exhibited no cross-reactivity or interference: BMP1, BMP2, BMP4, HGF, IL1 beta, IL1RA, IL2, IL4, IL5, sIL6R, IL8, IL10, IL12, IL13, IL15, IFN gamma, MMP2, MMP9, IL2R, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF. |
| Target Name | IL6 |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 25 pg/ml |
| Sample Type | Serum, plasma, cell lysate and tissue lysate. |
| Standard Range | 50 - 3200 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6% Inter-Assay CV: 9% |
| Alternate Names | B-cell stimulatory factor 2; CDF; HSF; BSF-2; Interferon beta-2; IL-6; IFNB2; CTL differentiation factor; Interleukin-6; HGF; Hybridoma growth factor; BSF2; IFN-beta-2 |
Application Instructions
| Assay Time | ~ 3.5 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL6 |
|---|---|
| Gene Full Name | interleukin 6 |
| Background | This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015] |
| Function | Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation. [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL6 antibodies; IL6 ELISA Kits; IL6 recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| PTM | N- and O-glycosylated. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG82643 Canine IL6 ELISA Kit standard curve image
ARG82643 Canine IL6 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82643 Canine IL6 ELISA kit User manual |
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