ARG82645
Canine IL4 ELISA Kit
Canine IL4 ELISA Kit for ELISA and Dog
Overview
| Product Description | ARG82645 Canine IL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Canine IL4 in serum and plasma. |
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| Tested Reactivity | Dog |
| Tested Application | ELISA |
| Specificity | The following recombinant canine proteins were tested and exhibited no cross-reactivity or interference: BMP1, BMP2, BMP4, HGF, IL1 beta, IL1RA, IL2, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IFN gamma, MMP2, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha and VEGF. |
| Target Name | IL4 |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 15 pg/ml |
| Sample Type | Serum and plasma. |
| Standard Range | 31 - 2000 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6% Inter-Assay CV: 9% |
| Alternate Names | BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1 |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL4 |
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| Gene Full Name | interleukin 4 |
| Background | The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
| Function | Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity). [UniProt] |
| Cellular Localization | Secreted. [UniProt] |
| Highlight | Related products: IL4 antibodies; IL4 ELISA Kits; IL4 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82645 Canine IL4 ELISA Kit standard curve image
ARG82645 Canine IL4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82645 Canine IL4 ELISA Kit User manual |
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