ARG82644
Canine IL1 alpha ELISA Kit
Canine IL1 alpha ELISA Kit for ELISA and Dog
Overview
| Product Description | ARG82644 Canine IL1 alpha ELISA Kit is an Enzyme Immunoassay kit for the quantification of Canine IL1 alpha in serum, plasma, cell lysate and tissue lysate. |
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| Tested Reactivity | Dog |
| Tested Application | ELISA |
| Specificity | The following recombinant canine proteins were tested and exhibited no cross-reactivity or interference: ApoA1, BMP1, BMP2, BMP3, BMP4, CCL4 / MIP1 beta, CRP, HSP27, IL1 beta, IL1RA, IL2, IL4, IL5, IL8, IL10, IL12, IL15, IL17C, IL21, IL23, IL2R, IFN gamma, PDGF, PLA2G7, prolactin, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF. |
| Target Name | IL1 alpha |
| Conjugation | HRP |
| Conjugation Note | Read at 450 nm. |
| Sensitivity | 1.3 pg/ml |
| Sample Type | Serum, plasma, cell lysate and tissue lysate. |
| Standard Range | 2.5 - 160 pg/ml |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: 6% Inter-Assay CV: 9% |
| Alternate Names | IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A |
Application Instructions
| Assay Time | ~ 3 hours |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | IL1A |
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| Gene Full Name | interleukin 1, alpha |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008] |
| Function | Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt] |
| Cellular Localization | Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. [UniProt] |
| Highlight | Related products: IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
Images (1) Click the Picture to Zoom In
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ARG82644 Canine IL1 alpha ELISA Kit standard curve image
ARG82644 Canine IL1 alpha ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG82644 Canine IL1 alpha ELISA kit User manual |
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