ARG83083
Canine / Feline Haptoglobin ELISA Kit
Canine / Feline Haptoglobin ELISA Kit for ELISA and Cat,Dog
Overview
| Product Description | ARG83083 Canine / Feline Haptoglobin ELISA Kit is an enzyme immunoassay kit for quantitative the Haptoglobin in dog and cat serum, plasma (citrate, heparin) or cell culture . |
|---|---|
| Tested Reactivity | Cat, Dog |
| Tested Application | ELISA |
| Target Name | Haptoglobin |
| Conjugation | HRP |
| Conjugation Note | Substrate: TMB and read at 450 nm. |
| Sensitivity | 22.9 ng/ml |
| Detection Range | 125 - 2000 ng/ml |
| Sample Type | serum, plasma (citrate, heparin) or cell culture. |
| Sample Volume | 100 µl |
| Precision | Intra-Assay CV: less than 8% Inter-Assay CV: less than 4% |
Application Instructions
| Assay Time | ~2 hour |
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Properties
| Form | 96 well |
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| Storage Instruction | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Haptoglobin |
|---|---|
| Background | This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. The protein encoded also exhibits antimicrobial activity against bacteria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014] |
| Function | As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. [UniProt] |
| Cellular Localization | Secreted |
| Highlight | Related products:
Haptoglobin antibodies; Haptoglobin ELISA Kits; |
| PTM | Disulfide bond, Glycoprotein |
Images (1) Click the Picture to Zoom In
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ARG83083 Canine / Feline Haptoglobin ELISA Kit curve image
ARG83083 Canine / Feline Haptoglobin ELISA Kit results of standard run with optical density reading at 450 nm
| Title | Download Link |
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| ARG83083 Canine / Feline Haptoglobin ELISA Kit |
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