ARG81124

Bovine Serum Albumin ELISA Kit

Bovine Serum Albumin ELISA Kit for ELISA and Bovine

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Component

Cat No Component Name Package Temp
ARG81124-001 Antibody-coated microplate 12 strips x 8 wells 4°C
ARG81124-002 10X Wash buffer 50 ml 4°C
ARG81124-003 Sample Diluent 70 ml (Ready-to-use) 4°C
ARG81124-004 Standard 1-6 1 ml X 6 vials (Ready-to-use) 4°C
ARG81124-005 Control 1 ml (200 ng/ml) 4°C
ARG81124-006 101X HRP-conjugate 0.3 ml 4°C
ARG81124-007 TMB substrate 15 ml (Ready-to-use) 4°C (Protect from light)
ARG81124-008 STOP solution 15 ml (Ready-to-use) 4°C

Overview

Product Description ARG81124 Bovine Serum Albumin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Bovine Serum Albumin in biological fluids.
Tested Reactivity Bov
Tested Application ELISA
Specificity ARG81124 Bovine Serum Albumin ELISA Kit reacts to Bovine Serum Albumin and it has been tested to cross-reacts to sheep serum albumin. And there is no significant cross-reactivity for 5 ng/ml –1000 ng/ml of Human serum albumin, Ovalbumin and rabbit albumin.
Target Name Albumin
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm
Sensitivity 15 ng/ml
Sample Type Biological fluids
Standard Range 15 - 500 ng/ml
Sample Volume 100 µl
Alternate Names allergen Bos d 6; Serum albumin; BSA; FDAH; ANALBA

Application Instructions

Assay Time ~3 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 280717 Bovine ALB

Swiss-port # P02769 Bovine Serum albumin

Gene Symbol ALB
Gene Full Name albumin
Background Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]
Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. [UniProt]
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PTM Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylated by FAM20C in the extracellular medium.
Acetylated on Lys-223 by acetylsalicylic acid.

Images (1) Click the Picture to Zoom In

  • ARG81124 Bovine Serum Albumin ELISA Kit standard curve image

    ARG81124 Bovine Serum Albumin ELISA Kit results of a typical standard run with optical density reading at 450 / 620 nm.

Specific References

Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice.

ELISA / Bovine

Takagaki Yuta et al.
Sci Rep.,  (2019)

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