ARG83431

Arginase Assay Kit

Datasheet

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96 wells

349.00

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Datasheet
Protocols

Overview

Product Description	ARG83431 Arginase Assay Kit can be used to measure Arginase in Serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids.
Tested Reactivity	Other
Tested Application	FuncSt
Target Name	Arginase
Conjugation Note	Read at 525 nm
Sensitivity	0.05 mmol/L
Sample Type	Serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids.
Standard Range	0.1 mmol/l - 3 mmol/l
Sample Volume	10 μl
Alternate Names	Arginase; E.C. 3.5.3.1; Canavanase; Arginine amidinase; L-Arginine amidinohydrolase; L-Arginase; Arginine transamidinase

Application Instructions

Assay Time	40 min

Properties

Form	96 well
Storage Instruction	Store the kit at 4°C and -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background	Arginase (E.C.3.5.3.1) is a mammalian enzyme which catalyzes the conversion of arginine to ornithine and urea. Arginase is considered as an enzyme responsible for the cyclic nature of urea cycle, since only the organisms containing arginase are able to carry out the complete urea cycle. Two distinct isoforms of mammalian arginase have been identified that are encoded by two separate genes. Type I arginase (arginase I) is located in the cytosol and is mainly expressed in liver. Type II arginase is located in the mitochondrial matrix and is expressed in extra-hepatic tissues.

Background

Arginase (E.C.3.5.3.1) is a mammalian enzyme which catalyzes the conversion of arginine to ornithine and urea. Arginase is considered as an enzyme responsible for the cyclic nature of urea cycle, since only the organisms containing arginase are able to carry out the complete urea cycle. Two distinct isoforms of mammalian arginase have been identified that are encoded by two separate genes. Type I arginase (arginase I) is located in the cytosol and is mainly expressed in liver. Type II arginase is located in the mitochondrial matrix and is expressed in extra-hepatic tissues.

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ARG83431 Arginase Assay Kit standard curve image

ARG83431 Arginase Assay Kit results of a typical standard run with optical density reading at 525 nm.