ARG30105
Apoptosis Marker Antibody Duo (Caspase3, PARP)
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Neuroscience antibody
Publication2
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG20001 | anti-Caspase 3 antibody [C33] | Mouse mAb | Hu | WB | 25 μg |
| ARG20041 | anti-PARP (cleaved) antibody | Rabbit pAb | Hu | IHC-P, WB | 25 μg |
Overview
| Product Description | The TUNEL assay has been commonly used as a detection method for identifying apoptotic cells. While useful, it can also yield highly variable results as some non-apoptotic nuclei showing high levels of RNA synthesis/splicing can also be labeled by TUNEL technique. Cleavage of Caspase 3 and PARP (Poly (ADP-ribose) Polymerase is a pre-requisite for cells who undergo final stage of cell death. The use of anti-cleaved caspase3 (ARG20003) and anti-cleaved PARP (ARG20041) antibodies adds a more specific weapon to the arsenal of techniques for confirming apoptosis in tissue specimens. |
|---|---|
| Target Name | Apoptosis Marker |
| Alternate Names | Apoptosis Marker antibody; Caspase 3 antibody; PARP (cleaved) antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Duo for Apoptosis Marker (Caspase3, PARP) |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Neuroscience antibody |
Images (4) Click the Picture to Zoom In
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ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 30 µg of HeLa cells untreated or treated with Staurosporine (1μM, overnight). The blots were stained with ARG20001 anti-Caspase 3 antibody [C33] at 1:500 dilution.
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ARG20041 anti-PARP (cleaved) antibody WB image
Western blot: 30 µg of HeLa untreated or treated with Staurosporine and stained with ARG20041 anti-PARP (cleaved) antibody at 1:500 dilution.
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ARG20001 anti-Caspase-3 antibody [C33] WB image
Western blot: HeLa cells treated with camptothecin (2 µM) stained with ARG20001 anti-Caspase-3 antibody [C33].
The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually. -
ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 20 µg of HeLa cell lysates stained with ARG20001 anti-Caspase 3 antibody [C33] at 2 µg/ml dilution.
Specific References
Moonlighting glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus gasseri inhibits keratinocyte apoptosis and skin inflammation in experimental atopic dermatitis
ARG20001: WB / Mouse
Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.
ARG20001: WB / Mouse
