ARG81239
Alkaline Phosphatase Activity Assay Kit (Colorimetric)
Alkaline Phosphatase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse
Component
| Cat No | Component Name | Package |
|---|---|---|
| ARG81239-01 | Alkaline Phosphatase Activity Substrate | 5 ml |
| ARG81239-02 | Standard (p-Nitrophenol) | 1 ml |
| ARG81239-03 | Cell Lysis Buffer | 20 ml |
| ARG81239-04 | Stop Solution (10X) | 10 ml |
Overview
| Product Description | ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) can be used to measure Alkaline Phosphatase activity in Human or Mouse cells. |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FuncSt |
| Target Name | Alkaline Phosphatase Activity |
| Conjugation | Un-conjugated |
| Conjugation Note | Read at 405 nm |
| Sample Type | Cultured cells (cell lysate) |
| Standard Range | p-nitrophenol: 0.3 - 19.2 nmol |
| Alternate Names | TNSALP; HOPS; EC 3.1.3.1; Alkaline phosphatase, tissue-nonspecific isozyme; TNAP; Alkaline phosphatase liver/bone/kidney isozyme; APTNAP; AP-TNAP |
Application Instructions
| Assay Time | ~ 1 hour |
|---|
Properties
| Form | Liquid |
|---|---|
| Storage Instruction | Store the kit at 2-8°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05186 Human Alkaline phosphatase, tissue-nonspecific isozyme Swiss-port # P09242 Mouse Alkaline phosphatase, tissue-nonspecific isozyme |
|---|---|
| Gene Symbol | ALPL |
| Gene Full Name | alkaline phosphatase, liver/bone/kidney |
| Background | There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypophosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010] |
| Function | This isozyme may play a role in skeletal mineralization. [UniProt] |
| PTM | N-glycosylated. |
Images (1) Click the Picture to Zoom In
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ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) standard curve image
ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) results of a typical standard run with optical density reading at 405 nm.
| Title | Download Link |
|---|---|
| ARG81239 Alkaline Phosphatase Activity Assay Kit (Colorimetric) User manual |
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