ARG82133

Alcohol Dehydrogenase Activity Assay Kit (Colorimetric)

Alcohol Dehydrogenase Activity Assay Kit (Colorimetric) for Functional study and Human,Mouse,Rat,All

Overview

Product Description ARG82133 Alcohol Dehydrogenase Activity Assay Kit (Colorimetric) can be used to measure Alcohol Dehydrogenase activity in serum, plasma, urine, tissue and culture media.
Tested Reactivity Hu, Ms, Rat, All
Tested Application FuncSt
Target Name ADH1B
Conjugation Note Read at 565 nm
Sensitivity 0.4 U/L (30 min reaction); 0.1 U/L (120 min reaction)
Detection Range 0.4 - 80 U/L for 30 min reaction
Sample Type serum, plasma, urine, tissue and culture media
Sample Volume 20 µl
Alternate Names HEL-S-117; ADH2; EC 1.1.1.1; Alcohol dehydrogenase subunit beta; Alcohol dehydrogenase 1B

Application Instructions

Application Note Please note that this kit does not include a microplate.
Assay Time 30 min

Properties

Form Liquid
Storage Instruction Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 125 Human ADH1B

Swiss-port # P00325 Human Alcohol dehydrogenase 1B

Gene Symbol ADH1B
Gene Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Background The protein encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
Cellular Localization Cytoplasm. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82133 Alcohol Dehydrogenase Activity Assay Kit (Colorimetric) enzyme kinetics graph

    Kinetics of 0 and 80 U/L Alcohol Dehydrogenase reaction, using ARG82133 Alcohol Dehydrogenase Activity Assay Kit (Colorimetric).

Title Download Link
ARG82133 Alcohol Dehydrogenase Activity Assay Kit (Colorimetric) User's manual download icon Download