ARG82014

Acid Phosphatase Assay Kit

Acid Phosphatase Assay Kit for Functional study and All species

Overview

Product Description ARG82014 Acid Phosphatase Assay Kit is a detection kit for the quantification of Acid Phosphatase Activity in urine, serum, plasma, tissue extracts, cell lysate and cell culture supernatants.
Tested Reactivity All
Tested Application FuncSt
Target Name Acid Phosphatase
Conjugation Note Read at 510 nm.
Sensitivity 0.04 mmol/L
Detection Range 0.04 - 4 mmol/L
Sample Type Urine, serum, plasma, tissue extracts, cell lysate and cell culture supernatants.
Sample Volume 10 µl
Alternate Names Low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; HAAP; Red cell acid phosphatase 1; EC 3.1.3.2; Low molecular weight cytosolic acid phosphatase; Adipocyte acid phosphatase; EC 3.1.3.48; LMW-PTPase

Application Instructions

Assay Time 15 min

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol ACP1
Gene Full Name acid phosphatase 1, soluble
Background The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]
Function Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity. [UniProt]
Cellular Localization Cytoplasm. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG82014 Acid Phosphatase Assay Kit typical data demonstration image

    ARG82014 Acid Phosphatase Assay Kit results of a typical data with optical density reading at 510 nm.

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