ARG81297
AST / Aspartate Transaminase Assay Kit
AST / Aspartate Transaminase Assay Kit for Functional study and Human,Mouse,Rat,Mammal
Publication1
Overview
| Product Description | ARG81297 AST / Aspartate Transaminase Assay Kit is a detection kit for the quantification of AST / Aspartate Transaminase in serum and plasma. |
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| Tested Reactivity | Hu, Ms, Rat, Mamm |
| Tested Application | FuncSt |
| Specificity | Aspartate aminotransferase (ASAT/AAT) facilitates the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate. And then oxaloacetate and NADH are converted to malate and NAD by malate dehydrogenase. Therefore, the decrease in NADH absorbance at 340 nm is proportionate to AST activity. |
| Target Name | AST / Aspartate Transaminase |
| Conjugation Note | Read at 340 nm. |
| Sensitivity | 2 U/l |
| Detection Range | 2 - 100 U/l |
| Sample Type | Serum and plasma. |
| Sample Volume | 20 µl |
| Alternate Names | Cysteine transaminase, cytoplasmic; cAspAT; GIG18; Glutamate oxaloacetate transaminase 1; cCAT; EC 2.6.1.3; Cysteine aminotransferase, cytoplasmic; ASTQTL1; AST1; EC 2.6.1.1; Transaminase A; Aspartate aminotransferase, cytoplasmic |
Application Instructions
| Application Note | Please note that this kit does not include a microplate. |
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| Assay Time | 10 min |
Properties
| Form | Liquid |
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| Storage Instruction | Store the kit at -20°C. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GOT1 |
| Gene Full Name | glutamic-oxaloacetic transaminase 1, soluble |
| Background | Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008] |
| Function | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. [UniProt] |
Images (1) Click the Picture to Zoom In
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ARG81297 AST / Aspartate Transaminase Assay Kit functional study image
Functional study: ARG81297 AST / Aspartate Transaminase Assay Kit results of a typical example of AST activity reading at 340 nm.
Specific References
Investigating the changes of some enzymes and metabolites of the Urea cycle in patients with type 2 diabetes treated with squalene
FuncSt / Human
| Title | Download Link |
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| ARG81297 AST / Aspartate Transaminase Assay Kit User's manual |
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