ARG30182
AMPK-ACC pathway Antibody Panel
Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Publication6
Component
| Cat No | Component Name | Host clonality | Reactivity | Application | Package |
|---|---|---|---|---|---|
| ARG51172 | anti-AMPK alpha 1/2 antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG51678 | anti-AMPK alpha 1 phospho (Thr183) + AMPK alpha 2 phospho (Thr172) antibody | Rabbit pAb | Hu, Ms, Rat | ICC/IF, IHC-P, WB | 50 μl |
| ARG51853 | anti-Acetyl CoA carboxylase 1 phospho (Ser79) antibody | Rabbit pAb | Hu | WB | 50 μl |
| ARG65351 | Goat anti-Rabbit IgG antibody (HRP) | Goat pAb | Rb | ELISA, IHC-P, WB | 50 μl |
Overview
| Product Description | AMP-activated protein kinase (AMPK) responds to hormonal and nutrient signals in the central nervous system and peripheral tissues, modulating food intake and whole-body energy homeostasis. The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia. Phosphorylates AMPKα1/2 at Thr172/Thr174 in the activation loop and this phosphorylation is required for AMPK activation. . In its activated state, AMPK phosphorylates serine residues 79, 1,200, and 1,215 of acetyl-coenzyme A carboxylase (ACC) which is reducing the activity of ACC. ACC is a potential target of anti-obesity drugs. Carling, D. (2004) Trends Biochem Sci 29, 18-24. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43. Woods, A. et al. (2003) J Biol Chem 278, 28434-42. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50. Warden, S.M. et al. (2001) Biochem J 354, 275-83. |
|---|---|
| Target Name | AMPK-ACC pathway |
| Alternate Names | AMPK-ACC pathway antibody; AMPK alpha 1/2 antibody; AMPK alpha 1/2 phospho (Thr183 / Thr172) antibody; Acetyl CoA carboxylase 1 phospho (Ser79) antibody |
Properties
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
|---|---|
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Full Name | Antibody Panel for AMPK-ACC pathway |
|---|---|
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
Images (7) Click the Picture to Zoom In
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ARG51172 anti-AMPK alpha 1/2 antibody WB image
Western blot: 30 µg of A549, HepG2, and Mouse liver cell line lysates stained with ARG51172 anti-AMPK alpha 1/2 antibody at 1:500 dilution.
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ARG51172 anti-AMPK alpha1/AMPK alpha2 antibody WB image
Western Blot: extracts from A549, HepG2 and 3T3 cells stained with anti-AMPK alpha1/AMPK alpha2 antibody ARG51172.
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ARG51678 anti-AMPK alpha 1 / AMPK alpha 2phospho (Thr183/Thr172) antibody WB image
Western Blot: extracts from C2C12 cells, treated with serum or calf intestinal phosphatase (CIP), stained with anti-AMPK alpha 1 / AMPK alpha 2(phospho Thr174/Thr172) antibody ARG51678.
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ARG51853 anti-Acetyl-CoA Carboxylase phospho (Ser79) antibody WB image
Western Blot: extracts from 293 cells untreated or treated with AICAR stained with anti-Acetyl-CoA Carboxylase (phospho Ser79) antibody ARG51853.
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ARG51172 anti-AMPK alpha1/AMPK alpha2 antibody IHC-P image
Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-AMPK alpha1/AMPK alpha2 antibody ARG51172 (left) or the same antibody preincubated with blocking peptide (right).
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ARG51172 anti-AMPK alpha1/AMPK alpha2 antibody ICC/IF image
Immunofluorescence: methanol-fixed HeLa cells stained with anti-AMPK alpha1/AMPK alpha2 antibody ARG51172.
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ARG51678 anti-AMPK alpha 1 / AMPK alpha 2phospho (Thr183/Thr172) antibody IHC-P image
Immunohistochemistry: paraffin-embedded human lung carcinoma tissue, stained with anti-AMPK alpha 1 / AMPK alpha 2(phospho Thr174/Thr172) antibody ARG51678).
Specific References
Expression, clinicopathological significance, and prognostic potential of AMPK, p-AMPK, PGC-1α, and TFAM in astrocytomas
ARG51172: IHC-P / Human
The role of CD36-Fabp4-PPARγ in skeletal muscle involves insulin resistance in intrauterine growth retardation mice with catch-up growth
ARG51172: WB / Mouse
Pentadecanoic acid promotes basal and insulin-stimulated glucose uptake in C2C12 myotubes
ARG51678: WB / Mouse
Amlexanox Enhances Temozolomide-Induced Antitumor Effects in Human Glioblastoma Cells by Inhibiting IKBKE and the Akt-mTOR Signaling Pathway
ARG51678: WB, IHC-P / Human
Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome.
ARG51172, ARG51678: WB / Human
Amlexanox Enhances Temozolomide-Induced Anti-Tumor Effects In Human Glioblastoma Cells By Inhibiting Ikbke And The Akt-mtor Signaling Pathway.
ARG51678: WB, IHC-P / Human
